Mientras más, mejor: análisis de interacciones proteicas mediante fluorescencia
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nov 29, 2024
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https://doi.org/10.30878/ces.v31n0a40
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Rubén Montalvo-Méndez
http://orcid.org/0000-0002-0469-5562
Gustavo Jiménez-Mejía
http://orcid.org/0000-0003-1773-1400
Diana Reséndez-Pérez
http://orcid.org/0000-0002-4709-7677
http://orcid.org/0000-0002-0469-5562
Gustavo Jiménez-Mejía
http://orcid.org/0000-0003-1773-1400
Diana Reséndez-Pérez
http://orcid.org/0000-0002-4709-7677
Resumen
En las últimas décadas, se han desarrollado nuevas estrategias que permiten el análisis de interacciones proteicas sin alterar la estructura celular. El objetivo de esta revisión es mostrar un panorama general para el estudio de las interacciones proteína-proteína. Se comparan diferentes técnicas fluorescentes tanto de complementación como de transferencia de energía y su combinación. Se presentan ventajas, desventajas y aplicaciones novedosas en cultivo celular y en organismos vivos con un enfoque en el uso de fluorescencia, luminiscencia y su combinación para la detección de interacciones multiproteicas. La implementación de estrategias con marcaje fluorescente y luminiscente, así como sus ingeniosas combinaciones, han desplazado las técnicas clásicas y permiten la detección más eficiente de complejos multiproteicos.
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MONTALVO-MÉNDEZ, Rubén; JIMÉNEZ-MEJÍA, Gustavo; RESÉNDEZ-PÉREZ, Diana.
Mientras más, mejor: análisis de interacciones proteicas mediante fluorescencia.
CIENCIA ergo-sum, [S.l.], v. 31, nov. 2024.
ISSN 2395-8782.
Disponible en: <https://cienciaergosum.uaemex.mx/article/view/20682>. Fecha de acceso: 04 dic. 2024
doi: https://doi.org/10.30878/ces.v31n0a40.
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Esta obra está bajo licencia internacional Creative Commons Reconocimiento-NoComercial-SinObrasDerivadas 4.0.
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Glöckner, N., zur Oven-Krockhaus, S., Rohr, L., Wackenhut, F., Burmeister, M., Wanke, F., ... & Harter, K. (2022). Three-fluorophore FRET Enables the Analysis of Ternary Protein Association in Living Plant Cells. Plants, 11(19), 2630. https://doi.org/10.3390/plants11192630
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Imani, M., Mohajeri, N., Rastegar, M., & Zarghami, N. (2021). Recent advances in FRET-Based biosensors for biomedical applications. Analytical biochemistry, 630, 114323. https://doi.org/10.1016/j.ab.2021.114323
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