Mientras más, mejor: análisis de interacciones proteicas mediante fluorescencia

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Rubén Montalvo-Méndez http://orcid.org/0000-0002-0469-5562
Gustavo Jiménez-Mejía http://orcid.org/0000-0003-1773-1400
Diana Reséndez-Pérez http://orcid.org/0000-0002-4709-7677

Resumen

En las últimas décadas, se han desarrollado nuevas estrategias que permiten el análisis de interacciones proteicas sin alterar la estructura celular. El objetivo de esta revisión es mostrar un panorama general para el estudio de las interacciones proteína-proteína. Se comparan diferentes técnicas fluorescentes tanto de complementación como de transferencia de energía y su combinación. Se presentan ventajas, desventajas y aplicaciones novedosas en cultivo celular y en organismos vivos con un enfoque en el uso de fluorescencia, luminiscencia y su combinación para la detección de interacciones multiproteicas. La implementación de estrategias con marcaje fluorescente y luminiscente, así como sus ingeniosas combinaciones, han desplazado las técnicas clásicas y permiten la detección más eficiente de complejos multiproteicos.

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MONTALVO-MÉNDEZ, Rubén; JIMÉNEZ-MEJÍA, Gustavo; RESÉNDEZ-PÉREZ, Diana. Mientras más, mejor: análisis de interacciones proteicas mediante fluorescencia. CIENCIA ergo-sum, [S.l.], v. 31, nov. 2024. ISSN 2395-8782. Disponible en: <https://cienciaergosum.uaemex.mx/article/view/20682>. Fecha de acceso: 04 dic. 2024 doi: https://doi.org/10.30878/ces.v31n0a40.
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